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Image Search Results
Journal: PLoS Pathogens
Article Title: Prion Uptake in the Gut: Identification of the First Uptake and Replication Sites
doi: 10.1371/journal.ppat.1002449
Figure Lengend Snippet: Peyer's patches were collected from wild-type 29/Ola mice 1 day after infection with ME7 prions. (A) Cellular organization of a Peyer's patch showing the LAMP1-positive compartments at the FAE and SED. (B) FAE enterocytes have large LAMP1-positive endosomes compared to enterocytes in the villi. (C and D) UEA-1 positive (red) M cells within the FAE. Note in (D) the large apical LAMP1+ endosomes are not present in M cells, but only in adjacent FAE enterocytes (arrowheads). N depicts the approximate location of nucleus in the epithelium (E) UEA-1 label is present only on FAE, whereas the neighbouring villus is negative. IgG (green) presents the endogenous mouse IgGs. (F and G) Cryo-immuno EM reveals that M cells in the FAE differ from enterocytes by having irregular microvilli that are UEA-1–positive (F, G). The diameter of the UEA-1–specific gold particles in (F) and (G) is 15 nm. Note in (F) the arrow points to a PrP-positive early endosomal vesicle in the UEA-1 negative enterocyte, whereas little PrP uptake is detectable in the UEA-1 positive M cell. PrP was detected with PrP-specific 6H4 monoclonal antibody directly conjugated to UltraSmall gold (PrP-usg) and visualized by silver enhancement. A larger high resolution version of is provided as a supplement . SED, subepithelial dome; GC, germinal centre; V, villus; M, M cells; E, enterocytes. Scale bars: (A) 80 µm; (B) 15 µm; (C) 10 µm; (D) 15 µm; (E) 20 µm; (F) 200 nm and (G) 200 nm.
Article Snippet: M cells were identified with
Techniques: Infection